Titlebook: Cerebrospinal Fluid Biomarkers; Charlotte E. Teunissen,Henrik Zetterberg Book 2021 Springer Science+Business Media, LLC, part of Springer

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Cerebrospinal Fluid Biomarkers978-1-0716-1319-1Series ISSN 0893-2336 Series E-ISSN 1940-6045

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https://doi.org/10.1007/978-3-642-34988-1This protocol describes sample preparation of CSF for proteomic analysis by LC-MS. The protocol can optionally be used in conjunction with quantification by the tandem mass tag (TMT) technique, which due to its high multiplexing capability is particularly useful in discovery proteomic studies when large cohorts are to be analyzed.

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Quantification of the Neurofilament Light Chain Protein by Single Molecule Array (Simoa) Assay,f disease activity in various neurological diseases. The development of a bead-based single molecule array (Simoa) assay for the neurofilament light chain provided a substantial improvement in sensitivity compared with conventional ELISA or electrochemiluminescence-based assays allowing reliable quantification in blood samples.

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CSF Cells: Cell Count, Cytomorphology, Cytology, and Immunophenotyping,logy provides additional important details that may be missed, if omitted, such as different stages of leukocyte activation, stages of phagocytosis, atypical cells, or tumor cells. While such level of detail may be not required for each and every sample, microscopic evaluation is recommended at leas

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,Oligoclonal Bands: Isoelectric Focusing and Immunoblotting, and Determination of κ Free Light Chainecal B cell activity. Immunoglobulins (Ig) and free light chains (FLC) which are both secreted by terminally differentiated B cells can be detected in the cerebrospinal fluid (CSF). As these proteins are not only present in the CSF in case of intrathecal inflammation, but also derive from blood by d