Titlebook: Cereal Genomics; Methods and Protocol Luis M. Vaschetto Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 High-t

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Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and ogical functions in all kingdoms of life. Recently, circRNAs have been identified in many plant species, including cereal crops, showing differential expression during stress response and developmental programs, which suggests their role in these process. In the following years, it is expected that

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Emerging Genome Engineering Tools in Crop Research and Breeding,netic material creates opportunities for rapid development of elite cultivars with desired traits. The plant genome can be altered in several ways, including targeted introduction of nucleotide changes, deleting DNA segments, introducing exogenous DNA fragments and epigenetic modifications. Targeted

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CRISPR/Cas9-Mediated Targeted Mutagenesis in Wheat Doubled Haploids,o conventional transformation methods. Additionally, the long generation time of crop plants poses a significant obstacle to effective application of gene editing technology, as it takes a long time to produce modified homozygous genotypes. The haploid single-celled microspores are an attractive tar

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Genetic Transformation of Protoplasts Isolated from Leaves of , and ,lized to screen potential sgRNA guides for CRISPR-mediated genome editing. However, little research has been conducted into the use of transient expression of protoplasts in . (a globally important pasture, hay, and amenity grass), and no studies have been conducted into . (a weed in cereal crops bu

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Transformation in the Rice Genome,ished by vigorous studies on the culture system and the elucidation of . transformation mechanisms. This section provides a detailed description of routine and efficient rice transformation protocols by .. This method uses mature seeds as a material and can be applied to many . and some other variet

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In Vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isofo by reversible phosphorylation in vascular plants. The phosphorylation site on a PEPC molecule is conserved not only among isoforms but also across plant species. An anti-phosphopeptide antibody is a common and powerful tool for detecting phosphorylated target proteins with high specificity. We gene

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Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/223261.jpg

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Cereal Genomics978-1-4939-9865-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/978-3-658-25677-7diated by DNA methylation. Here we describe a bioinformatics protocol to analyze gene expression levels using RNA-seq data that allow us to identify candidate genes to be tested by bisulfite assays. The candidate methylated genes are usually those that are low expressed in a particular condition or developmental stage.