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Titlebook: Cell Viability Assays; Methods and Protocol Daniel F. Gilbert,Oliver Friedrich Book 2017 Springer Science+Business Media LLC 2017 Misestima

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發(fā)表于 2025-3-21 19:21:08 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Cell Viability Assays
副標題Methods and Protocol
編輯Daniel F. Gilbert,Oliver Friedrich
視頻videohttp://file.papertrans.cn/223/222897/222897.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Cell Viability Assays; Methods and Protocol Daniel F. Gilbert,Oliver Friedrich Book 2017 Springer Science+Business Media LLC 2017 Misestima
描述.This volume provides an overview of commonly used methods and protocols for cell fitness indicators. Chapters detail biochemical, fluorescence and luminescence-based strategies, computational, and label-free methodologies for assaying cellular viability by means of e.g. viscoelastic properties, impedance and multiphoton microscopy. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...Authoritative and practical, .Cell Viability Assays: Methods and Protocols .aims to ensure successful results in the further study of this vital field..
出版日期Book 2017
關鍵詞Misestimation; enzyme; cell proliferation; Phenotyping; cell imaging
版次1
doihttps://doi.org/10.1007/978-1-4939-6960-9
isbn_softcover978-1-4939-8352-0
isbn_ebook978-1-4939-6960-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2017
The information of publication is updating

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K. -H. Hoffmann,E. J?rn,E. Sch?fer,H. Weberculate dose-response. The methods would be useful to researchers in preclinical and translational research planning to move away from simplistic monolayer studies and explore 3D spheroid screens for drug safety and efficacy without substantial investment in money or time.
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https://doi.org/10.1007/BFb0077985 and resazurin. They are applicable for adherent or suspended cell lines, easy to perform, and comparably economical. Detailed protocols and notes for easier handling and avoiding pitfalls are enclosed to each assay.
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Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness,to make the inner mitochondrial membrane permeable for protons and allow maximum electron flux through the electron transport chain, and rotenone and antimycin A—to inhibit complexes I and III, respectively. This chapter describes the protocol of OCR assessment in the culture of primary myotubes obtained upon satellite cell fusion.
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Book 2017minescence-based strategies, computational, and label-free methodologies for assaying cellular viability by means of e.g. viscoelastic properties, impedance and multiphoton microscopy. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to th
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