Titlebook: Cell Migration in Three Dimensions; Coert Margadant Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

Guileless

Visualization of Exosome Release and Uptake During Cell Migration Using the Live Imaging Reporter pHigration is critical for investigation of these mechanisms. Exosomes can be visualized by direct labeling with fluorescent dyes or by tagging intrinsic markers with fluorescent proteins for live imaging. Due to several limitations of fluorescent dye-labeled exosomes, we created two bright geneticall

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Dissecting Collective Cell Behavior in Migrating Testis Myotubes in animal models have been established to analyze how chemical and mechanical stimuli shape the behavior of single cells into tissues and organs. At present, there are still only a few model systems that allow to genetically dissect underlying molecular mechanisms driving cell motility during tissue mo

AXIOM

Whole-Mount In Situ Hybridization for Detection of Migrating Zebrafish Endodermal Cellsation that gives rise to the internal organs. Recent years have seen renewed interest in the mechanisms underlying the specification and migration of endodermal progenitor cells. The zebrafish is a well-established, accessible, and powerful model to study this cell population. Zebrafish endodermal c

現(xiàn)實

Live-Imaging Analysis of Epithelial Zippering During Mouse Neural Tube Closuremilar to the travel of a zip fastener, to ultimately ensure closure of an opening. A comparable process can be observed during . dorsal closure and mammalian wound healing, while zippering is employed by numerous organs such as the optic fissure, palatal shelves, tracheoesophageal foregut, and presu

Lucubrate

Time-Lapse Imaging and Morphometric Analysis of Tracheal Development in l mechanisms of morphogenesis and pattern formation. Observation of live tissues or whole-mount fixed specimens has emerged as the method of choice for identifying and quantifying specific cellular and tissular structures within the organism. In both cases, cell and subcellular structure identificat

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A Guide Toward Multi-scale and Quantitative Branching Analysis in the Mammary Glandown during puberty, during which actively proliferating terminal end buds repeatedly elongate and bifurcate to form the basic structure of the ductal tree. Mammary ducts consist of a basal and luminal cell layer with a multitude of identified sub-lineages within both layers. The understanding of how

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Analysis of Integrin-Dependent Melanoblast Migration During Developmentand enteric nervous systems, pigment-producing skin cells known as melanocytes, and many craniofacial structures. Melanoblasts, the precursors of melanocytes, are derived from the so-called trunk neural crest cells. These cells delaminate and migrate along a dorsolateral pathway to colonize their fi

Exposition

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Analysis of Energy-Driven Leader-Follower Hierarchy During Collective Cancer Cell Invasiongration makes cancer cell clusters more invasive and metastatic than individual cells. A cohesive cohort of cancer cells can have many advantages over individual cells, including more efficient bioenergetics that have been recently identified. Minimization of bioenergetic costs during collective cel