Titlebook: Cell Migration; Methods and Protocol Alexis Gautreau Book 2018 Springer Science+Business Media, LLC 2018 mammalian cells.High throughput an

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1064-3745 ation advice from the experts.This volume covers various assays and techniques that have been developed to study and characterize the cell migration in vitro, ex vivo, and in vivo. The chapters in this book present readers with the latest protocols to observe, quantify, and control cell migration. S

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Advertising and Advertising Claims Over Time one model, MGE-derived interneuron precursors are cultured and migrate on a flat synthetic substrate. In the other model, fluorescent MGE-derived interneuron precursors migrate on a monolayer of dissociated cortical cells. In both models, cell movements can be recorded by time-lapse microscopy for dynamic analyses.

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https://doi.org/10.1007/b137137describe a complete procedure allowing in vivo imaging methods, at high throughput and spatiotemporal resolution, to assess the behavior of circulating tumor cells (CTCs) in an experimental metastasis assay. This protocol provides access, for the first time, to the earliest steps of tumor cell seeding during metastasis formation.

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https://doi.org/10.1007/b137137aring behavior of these cells to control or neighboring cells allows determination of the role of the candidate gene in cell migration. We describe the procedure, focusing on one specific cell population during gastrulation, but it can easily be adapted to other cell populations and other migration events during early embryogenesis.

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https://doi.org/10.1007/b137137ies, including quantifications of progeny position, genetic screening strategies, preparation of migration mutants or transgenic worms expressing related fluorescent proteins, multipositional time-lapse tracking of Q cell migration using confocal microscopy and image analyses of single cell movements and dynamics.