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Titlebook: Cell Cycle Synchronization; Methods and Protocol Gaspar Banfalvi Book 2011 Springer Science+Business Media, LLC 2011 Bacteria.Mammalian cel

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書目名稱Cell Cycle Synchronization
副標(biāo)題Methods and Protocol
編輯Gaspar Banfalvi
視頻videohttp://file.papertrans.cn/223/222794/222794.mp4
概述Offers practical lab protocols for synchronizing mammalian, plant, yeast, fungal, and even bacterial cells.Serves a wide variety of scientists, from cell biologists to medical students to pharmacologi
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Cell Cycle Synchronization; Methods and Protocol Gaspar Banfalvi Book 2011 Springer Science+Business Media, LLC 2011 Bacteria.Mammalian cel
描述.To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe.? In .Cell Cycle Synchronization: Methods and Protocols., experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events.? Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the “arrest-and-release” approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle.? Written in the highly successful .Methods in Molecular Biology?. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols,
出版日期Book 2011
關(guān)鍵詞Bacteria; Mammalian cells; Model systems; Plant cells; Regulatory mechanisms; Stability; Storage condition
版次1
doihttps://doi.org/10.1007/978-1-61779-182-6
isbn_softcover978-1-4939-6194-8
isbn_ebook978-1-61779-182-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2011
The information of publication is updating

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Overview of Cell Synchronization,cytofluorometric purification), chemical blockade, (3) synchronization of embryonic cells, (4) synchronization at low temperature, (5) comparison of cell synchrony techniques, (6) synchronization of unicellular organisms, and (7) the effect of synchronization on transfection.
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Cell Synchronization by Inhibitors of DNA Replication Induces Replication Stress and DNA Damage Resorylation of histone H2AX-.139, ATM/ATR substrate on . at SQ/TQ cluster domains as well as . (ATM) protein kinase in cells treated with inhibitors of DNA replication. Phosphorylation of these proteins is detected in individual cell immunocytochemically with phospho-specific antibody (Ab) and measure
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Flow Cytometry Developments and Perspectives in Clinical Studies: Examples in ICU Patients,e anergy, secondary nosocomial infection, and death in critically ill patients. Finally, the measurement of an increased CD4.CD25.CD127. regulatory T cell percentage may represent a reliable marker for the diagnosis of lymphocyte dysfunctions in these patients. These stainings can be performed using
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Pluralism Versus Monism in Cultural Patternutriation early log S phase cell populations are most suitable where most of the cells are in G1 and S phase (>80%). Protocols for the synchronization of nuclei of murine pre-B cells and high-resolution centrifugal elutriation of CHO cells are given. The verification of purity and cell cycle positio
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