Titlebook: Cancer Vaccines; Methods and Protocol Michael J.P. Lawman,Patricia D. Lawman Book 2014 Springer Science+Business Media New York 2014 Cancer

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https://doi.org/10.1007/978-981-10-3872-3roaches have been used to obtain and load tumor antigens in DC. One such technique is to use whole tumor cell lysate from one or more tumor cell lines of the tumor type to be treated. The advantage of applying this method is that it provides a large spectrum of tumor antigens. However, some consider

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https://doi.org/10.1007/978-981-10-3872-3ls. Therefore, DC are being extensively evaluated as vehicles for antigen delivery in immunotherapies for the treatment of patients with cancer. Many techniques have been used to load DC with tumor-associated antigens (TAA), including pulsing with synthetic peptides that represent T cell epitopes. T

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https://doi.org/10.1007/978-981-10-3872-3r antigens is a strategy to arm DC against tumor without human leukocyte antigen (HLA) restriction. Furthermore, this approach allows the presentation of a full antigen range to the immune system. We describe the methods to obtain whole antigens from autologous tumor tissues in order to load DC gene

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https://doi.org/10.1007/978-981-10-3872-3essential components like genes encoding cytokines, chemokines, and other molecules or stimulation with recombinant cytokines is a potential method for designing an effective tumor vaccine protocol. Here, we describe the stimulation of purified splenic- or bone marrow-derived DC with recombinant int

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VAR Processes with Parameter Constraintscapable of stimulating or targeting dendritic cells (DC), the most important antigen-presenting cells for inducing CD8. cytotoxic T lymphocytes (CTL) which can efficiently kill tumor cells expressing the tumor antigen. The DC-stimulating or DC-targeting proteins, including granulocyte/macrophage col

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Estimation of Vector Error Correction Modelsthe unique capacity to stimulate an antigen-specific T-cell responses by na?ve T cells. Adenoviruses (Ad) have high transduction efficiency for many cell types including cells of hematopoietic origin independent of their mitotic status, and replication-defective Ad have demonstrated a safety profile

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