Titlebook: Cancer Cell Biology; Methods and Protocol Sherri L. Christian Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive li

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Nicole Yang,Jonathan Scott Coblyno system, we prepared whole cell lysates from HER2-overexpressing breast cancer cell lines, treated or not with the tyrosine kinase inhibitor, lapatinib, in the presence and absence of IFN-γ. Here we describe the protocol whereby proteins in the lysates were separated by SDS-PAGE, electrophoreticall

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The Hierarchical Nature of Brain Dynamics,uring cellular metabolism is therefore central to understanding the cellular physiology of cancer cells. The Seahorse XFe Analyzer series allows real-time measurement of cellular metabolism. In the basic assay, two parameters, the oxygen consumption rate (OCR) and the extracellular acidification rat

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The Hierarchical Nature of Brain Dynamics,olution. The Fluidigm C1 is an automated system for preparing complementary deoxyribonucleic acid (cDNA) samples that have been synthesized and preamplified from single cells for next-generation sequencing (NGS) analysis. Herein, we detail a workflow and protocol for scRNA-seq analysis of 3′-end enr

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Culturing Suspension Cancer Cell Lines,c cells that exist in the circulation of multicellular animals such as mouse and humans. Generally, cell lines derived from the blood such as lymphocytes, megakaryocyte, and neutrophils grow in suspension. These cell lines can be used for experimental studies to understand the biology/biochemistry o

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Breast Cancer Xenograft Murine Models,d immunocompromised mouse models are used depending on the scope of the study. Immunocompetent models allow the study of the impact of the immune system in murine models of mammary cancer, while immunodeficient mice serve as ideal host organisms to understand the behavior of human breast cancers wit