Titlebook: Calcium Signaling Protocols; David G. Lambert Book 19991st edition Springer Science+Business Media New York 1999

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Measurement of Inositol(1,4,5)trisphosphate Using a Stereospecific Radioreceptor Mass AssayP. receptor on the smooth endoplasmic reticulum, causing a conformational change that opens the intrinsic calcium channel in the receptor, thus allowing the efflux of calcium ions from the intracellular stores (.). For further details, see the reviews by Berridge (.) and Furuichi and Mikoshiba (.).

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Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay,5)P.” (.). An alternative approach involves disruption of the plasma membrane and preparation of microsomes from the intracellular vesicular Ca. stores (.,.), however, these preparations exhibit a loss of Ins(1,4,5)P. responsiveness compared to cells. The author will describe a .Ca.-release assay u

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