Titlebook: CRISPR-Cas Methods; M. Tofazzal Islam,Pankaj K. Bhowmik,Kutubuddin A. Book 2020 Springer Science+Business Media, LLC, part of Springer Na

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Correction to: A Procedure to Design Guide RNA, Assemble Fragments, and Detect Mutation for Genome

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CRISPR-Cas9-Mediated Genome Editing of the Model Grass Species ,thin a single nucleotide difference to the wild-type sequence) to quickly ascertain the number of bases inserted or deleted on each allele of the targeted gene, which can then be followed by sequencing homozygous progeny of the initial regenerated transformants.

加強防衛(wèi)

An Optimized RNA-Guided Cas9 System for Efficient Simplex and Multiplex Genome Editing in Barley (,n a single T-DNA construct via stable .-mediated transformation of immature embryos. Mutations induced at the target loci are detected in T. plants, and homozygous transgene-free mutants segregate in the T. and T. generations. Here, we present methods and strategies for the induction of single and m

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Delivery of Cas9/sgRNA RNP into Wheat Microspores Using Synthetic CPP for Genome Editing and Gene Eer non-transgenic means to genome editing. In this chapter, we describe a novel method for delivery of in vitro purified Cas9/sgRNA complexes into wheat microspores for generation of homozygous edited plants. We provide guidelines for purification of Cas9 protein from bacterial culture, in vitro tra

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CRISPR-Cas9-Mediated Gene Editing in Wheat: A Step-by-Step Protocol,protocol for the implementation of CRISPR-mediated wheat gene editing and trait improvement. We begin with gRNA designing using online tools, followed by assembling CRISPR constructs or ribonucleoprotein (RNP) complex and in vitro and in vivo gRNA validation using wheat mesophyll protoplasts. Furthe

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Noch einmal: Geschichte in Schichtgesteinen, proteins) has revolutionized our ability to manipulate DNA and RNA sequences in vitro, ex vivo, and in vivo. In this introductory chapter, we present a brief overview of basics of CRISPR-Cas-mediated genome editing and different orthologues and engineered versions of Cas protein with altered specif

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Aus der Geschichte der wirbellosen Tiere, a powerful tool for improving abiotic and biotic stress tolerance and increasing yield in rice. Valuable agronomical traits and functional study of a novel gene can be obtained by targeting single or multiple mutations in the rice genome. Here we describe the sgRNA design, cloning, and assembly of

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