Titlebook: CRISPR Gene Editing; Methods and Protocol Yonglun Luo Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 CRISPR-C

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Conditional Gene Knockout in Human Cells with Inducible CRISPR/Cas9ut experiments using CRISPR/Cas9 are very valuable, these are not well suited to study stage-specific gene function in dynamic situations such as development or disease. Here we describe a CRISPR/Cas9-based OPTimized inducible gene KnockOut method (OPTiKO) for conditional loss-of-function studies in

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CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells of transgenes in Chinese hamster ovary (CHO) cells. Targeted gene integration allows the generation of stable cell lines with a controlled and predictable behavior, which is an important feature for the rational design of cell factories aimed at the large-scale production of recombinant proteins. H

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Yonglun LuoIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/220555.jpg

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