Titlebook: Biotechnology of Plant Secondary Metabolism; Methods and Protocol Arthur Germano Fett-Neto Book 2016 Springer Science+Business Media New Yo

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Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/188777.jpg

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Biotechnology of Plant Secondary Metabolism978-1-4939-3393-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/BFb0058562lants, fuelled by the power of next-generation sequencing to identify novel candidate genes. Here, we describe a simple and effective method for isolation and transformation of protoplasts, and illustrate its application to several plant materials.

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Book 2016y protocols, and tips on troubleshooting and avoiding known pitfalls...Practical and cutting-edge, .Biotechnology of Plant Secondary Metabolism: Methods and Protocols .is a great resource for scientists of interdisciplinary fields--plant science, plant physiology, pharmacy, molecular biology, bioche

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https://doi.org/10.1007/3-540-69151-0o select highly expressible amiRNA candidates for stable genetic transformation. Here, we describe the methods for design of amiRNA candidates with perfect or almost perfect base-pairing to the target gene or gene groups, incorporation of amiRNA candidates in miR168a gene backbone by one step invers

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