Titlebook: Biophysical approaches to translational control of gene expression; Jonathan D. Dinman Book 2013 Springer Science+Business Media New York

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書目名稱Biophysical approaches to translational control of gene expression影響因子(影響力)學科排名




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書目名稱Biophysical approaches to translational control of gene expression讀者反饋學科排名

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Structural Analyses of the Ribosome by Chemical Modification Methods,to both RNA structure and dynamics. This chapter describes past and current methods of performing structural analysis of RNA using chemical modification techniques. We explore both radioactive and nonradioactive methodologies, a variety of reagents available to probe for different types of informati

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Methods for Studying the Interactions of Translation Factors with the Ribosome, decades toward this goal. Cross-linking and footprinting were among the first methods used to probe the interactions of translation factors with the ribosome and the structures of ribosomal complexes. Since publication of the first high-resolution structures, these methods have continued to provide

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Riboproteomic Approaches to Understanding IRES Elements,t initiation mechanism that operates in the vast majority of mRNAs and the internal ribosome entry site (IRES)-dependent mechanism. IRES elements, first discovered in picornaviruses, are .-acting RNA sequences that form secondary and tertiary structures and recruit the translation machinery using a

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Investigating RNAs Involved in Translational Control by NMR and SAXS,mine molecular structure. Here, we review the use of NMR and SAXS for the structural analysis of RNA molecules, with a focus on RNAs involved in translational control. Practical considerations, experimental requirements, and data analysis are discussed. We also review a recently developed hybrid app

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,Analyses of RNA–Ligand Interactions by Fluorescence Anisotropy,s. By manipulating assay design, it is possible to extract equilibrium binding constants down to the low or even sub-nanomolar range, as well as measure reaction on- and off-rates, changes in enthalpy and entropy, and RNA-binding site size. These tools are invaluable for comparing and contrasting po

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