Titlebook: Biomembrane Protocols; I. Isolation and An John M. Graham,Joan A. Higgins Book 1993 Humana Press 1993 Biomembran.Peroxisom.Western Blottin

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https://doi.org/10.1007/978-3-0346-0439-0g homogenate, and then identified unambiguously. The early chapters of this volume, ., are concerned with this fractionation process. Later chapters are concerned with the determination of composition, whereas in the second volume, ., the methods associated with architecture and function predominate.

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Isolation of Plasma Membrane Sheets and Plasma Membrane Domains from Rat Liver,in is specialized for the transport of bile acids and is separated from the basolateral domain by tight junctions, which prevent lateral diffusion of membrane proteins and outer leaflet lipids. Biochemical analysis of hepatocyte PM constituents requires the isolation of substantial amounts of this organelle.

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Isolation of Cholinergic-Specific Synaptosomes by Immunoadsorption,high yield under conditions that do not expose organelles to unusual media or to osmotic shock. Immunoisolation techniques also have the advantage of being applicable to situations where only a small amount of cell material is available, e.g., tissue culture cells.