Titlebook: Biomembrane Protocols; II. Architecture and John M. Graham,Joan A. Higgins Book 1994 Springer Science+Business Media New York 1994

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https://doi.org/10.1007/978-3-642-55426-1ble in permeable vesicles or only accessible in inside-out vesicles are considered to be in the inner leaflet of the membrane bilayer. However, the characteristics of the membrane preparation under investigation and the enzyme probe are critical in these experiments. The following criteria are important.

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Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination,e cell. This asymmetry (polarity) extends on a biochemical level to the membrane proteins of the apical and basolateral plasma membrane. Indeed, many of the polarized functions of epithelia are mediated by membrane proteins enriched or restricted to one particular cellular domain (.,.).

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Determination of the Transverse Topography of Membrane Lipids Using Enzymes and Covalent Labels as ble in permeable vesicles or only accessible in inside-out vesicles are considered to be in the inner leaflet of the membrane bilayer. However, the characteristics of the membrane preparation under investigation and the enzyme probe are critical in these experiments. The following criteria are important.

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Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry,ution is a prerequisite for understanding the functional properties of membranes. This chapter will deal with a recently developed, noninvasive technique to assess the distribution of phospholipids over the two leaflets of biological membranes with emphasis on the distribution of negatively charged phospholipids (.,.).

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