Titlebook: Bioluminescence; Methods and Protocol Preston B. Rich,Christelle Douillet Book 2009Latest edition Humana Press 2009 ATP.Calcium.Colon.Expre

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,Analysis of Protein–Protein Interactions Using Bioluminescence Resonance Energy Transfer, these fusion proteins is then detected when they are in close proximity, indicative of association either directly or as part of a complex. BRET is particularly useful for real-time monitoring of ligand-modulated interactions as dynamic changes in protein complex assembly can be observed in a live cell environment.

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Preston B. Rich,Christelle DouilletProvides an easily accessible reference volume for a wide range of bioluminescence assays and applications written by leading researchers in the field.Comprehensive guide to developing, validating, ru

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Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/187775.jpg

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Aufbau der Arbeit und Vorgehensweisee discovery of luciferin and luciferases, molecular biology techniques allowed the cloning of several luciferases and photoproteins. Among most used BL reporters, we find firefly and click-beetle luciferases, bacterial luciferase, ., ., and . luciferases, and calcium-activated photoproteins. Accordi

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Arbeitsdefinition von Komplexit?tesearch, cell transplantation, and monitoring of infectious disease in which bioluminescence intensity is correlated with other metrics. However, bioluminescence measurements can be influenced by a number of factors, among them source location, tissue optical properties, and substrate availability a