Titlebook: Bioluminescence; Methods and Protocol Sung Bae Kim Book 2016Latest edition Springer Science+Business Media New York 2016 luciferase.optical

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Zusammenfassung und Schlussfolgerungeneptionally suitable for deep tissue imaging in living animals owing to its long wavelength emission light. Herein, we report the experimental detail of bioluminogenic imaging of aminopeptidase N activity both in vitro and in vivo.

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Michael Snowden,Jamie P. Halsallin exhibits pH dependence in vitro. When expressed in either yeast or mammalian cells, pHlash reports basal pH and cytosolic acidification. In this chapter, we describe an in vitro characterization of pHlash, and also in vivo assays including in yeast cells and in HeLa cells using pHlash as a cytoplasmic pH indicator.

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Bioluminogenic Imaging of AminopeptidaseN In Vitro and In Vivoeptionally suitable for deep tissue imaging in living animals owing to its long wavelength emission light. Herein, we report the experimental detail of bioluminogenic imaging of aminopeptidase N activity both in vitro and in vivo.