Titlebook: Bacterial and Archaeal Motility; Tohru Minamino,Makoto Miyata,Keiichi Namba Book 2023 The Editor(s) (if applicable) and The Author(s), und

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High-Resolution Rotation Assay of the Bacterial Flagellar Motor Near Zero Loads Using a Mutant Havina wide range of external load, but the elasticity of the axial parts of the flagellum, such as the hook and filament, limits the spatiotemporal resolution. In this chapter, we describe a bead assay optimized for the analysis of the flagellar motor dynamics at near zero load.

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Book 2023ective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and thorough, .Bacterialand Archaeal Motility. is the ideal reference for researchers working in this vital area of microbiology..

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Purification of the Transmembrane Polypeptide Channel Complex of the , Flagellar Type III Secretion embrane export gate complex, which acts as a proton/protein antiporter that couples proton flow with flagellar protein export, and a cytoplasmic ATPase ring complex, which works as an activator of the export gate complex. Three transmembrane proteins, FliP, FliQ, and FliR, form a core structure of t

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