Titlebook: Bacterial Pangenomics; Methods and Protocol Alessio Mengoni,Marco Galardini,Marco Fondi Book 2015 Springer Science+Business Media New York

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Eike Bohlken PD Dr.,Christian Thies PD Dr. beginning of the twenty-first century. Thanks to the possibility to produce large amount of sequence data, these tools are going to completely substitute other high-throughput technologies. Moreover, the large applications of NGS protocols are increasing the genetic decoding of biological systems t

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https://doi.org/10.1007/978-3-531-92452-6ches spanning entire genomes, with the latest instruments capable of producing hundreds of gigabases of data in a single sequencing run. Illumina’s NGS instruments powerfully combine the flexibility of single reads with short- and long-insert paired-end reads, and enable a wide range of DNA sequenci

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https://doi.org/10.1007/978-3-531-92452-6ss, there is the need to pay attention to several crucial steps in order to obtain high-quality and reproducible data from PM, such as the choice of the Dye mix, the type and concentration of the carbon source in metabolic experiments, the use of a buffered medium. A systematic research of auxotroph

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Wolfgang Schroeder,Bernhard We?elsan display different lifestyles and it is still not well known to what extent the core pangenome plays a role in the divergence of lifestyles between the two organisms. Here, we present a procedure for uncovering the conservation and divergence of gene expression by using large expression compendia.