Titlebook: Bacterial Amyloids; Methods and Protocol Véronique Arluison,Frank Wien,Andrés Marcoleta Book 2022 The Editor(s) (if applicable) and The Aut

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Anti-Amyloid Drug Screening Methods Using Bacterial Inclusion Bodies,iseases such as Alzheimer’s and Parkinson’s diseases. The formation of insoluble protein aggregates with amyloid conformation inside bacteria, namely, in bacterial inclusion bodies, offers the possibility to use bacteria as simple models to study amyloid aggregation processes and potential effects o

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Design and Testing of Synthetic Catalytic Amyloids Based on the Active Site of Enzymes,ructural organization of amyloid fibrils can be exploited for the design of novel nanomaterials with emergent properties. Recent works have demonstrated that the functional features of the active site of enzymes can be partially recreated using this fold as a scaffold to develop catalytically active

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Assessment of Intracellular Amyloid Formation in Fixed and Live Bacteria Using Fluorescence Microscisms have harnessed amyloidogenesis to perform diverse biological processes. These functional amyloids, some of them secreted and others intracellular, require that the producing cells keep aggregation under control in the cytoplasm upon protein translation, preventing their inherent toxicity. Thus,

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Identification of Aggregation-Prone and Gatekeeper Residues in Bacterial Amyloids Using Site-Directgical processes. In this context, it is highly relevant to understand how amyloidogenesis is modulated by relevant factors, including key amino acids promoting or preventing aggregation. This chapter describes a methodology to identify critical residues for amyloid formation in bacterial proteins, b

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