Titlebook: Auditory and Vestibular Research; Methods and Protocol Bernd Sokolowski Book 2009 Humana Press 2009 cells.gene expression.gene transfer.gen

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Lineage Analysis of Inner Ear Cells Using Genomic Tags for Clonal Identificationmigrational boundaries of individual clones within the inner ear. This chapter details the basic methods for performing lineage analysis of the inner ear using replication-defective retroviral vectors in chicken embryos. Protocols are provided for generating avian retroviruses pseudotyped with vesic

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Electroporation-Mediated Gene Transfer to the Developing Mouse Inner Eardges cinch together to establish a fluid-filled sac called the otic vesicle or otocyst. The progenitor cells lining the otocyst lumen will give rise to sensory and non-sensory cells of the inner ear. These formative stages of inner ear development are initiated during the first week of postimplantat

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Isolation of Sphere-Forming Stem Cells from the Mouse Inner Earhearing loss as a result of either ototoxic insult or the aging process. Coincidently, with this inability to regenerate lost hair cells, the adult cochlea does not appear to harbor cells with a proliferative capacity that could serve as progenitor cells for lost cells. In contrast, adult mammalian

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Molecular Biology of Vestibular Schwannomasr schwannoma research is the development of new treatment options; however, such options have not yet been established. A fundamental understanding of the underlying molecular events leading to tumor formation began when mutations in the . tumor suppressor gene were identified in vestibular schwanno

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