Titlebook: Antimicrobial Therapies; Methods and Protocol Carlos Barreiro,José-Luis Barredo Book 2021 Springer Science+Business Media, LLC, part of Spr

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A User Guide for the Identification of New RiPP Biosynthetic Gene Clusters Using a RiPPER-Based Worklow which integrates the functionalities of RiPPER with existing bioinformatic tools into a complete genome mining strategy. This includes some key updates to RiPPER (updated to version 1.1), which substantially simplify implementing this workflow.

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1064-3745 expertsAntimicrobial resistance will become a global health threat since antimicrobial treatments continue at the forefront of the defense against microbial infections. To respond to the issue, this detailed book explores vital methodologies currently in use to advance our understanding of antibiot

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https://doi.org/10.1007/978-3-642-02723-9liferative test oriented to the discovery of new cytotoxic compounds synthesized by marine bacteria. In addition to the discovery of new bioactive secondary metabolites, this protocol provides a high-throughput isolation and screening platform for discarding no bioactive strains during the first steps of the drug discovery process.

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Dilution-to-Extinction Platform for the Isolation of Marine Bacteria-Producing Antitumor Compoundsliferative test oriented to the discovery of new cytotoxic compounds synthesized by marine bacteria. In addition to the discovery of new bioactive secondary metabolites, this protocol provides a high-throughput isolation and screening platform for discarding no bioactive strains during the first steps of the drug discovery process.

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Screening Fermentation and Extract Generation The generation of extracts is based on a solvent mixture that could be varied in order to improve the extraction of products with different polarity. These extracts are then stored in a 96-well microtube format to facilitate their usage for the screening of bioactive compounds.