Titlebook: Antifungal Drug Resistance; Methods and Protocol Damian J. Krysan,W. Scott Moye-Rowley Book 2023 The Editor(s) (if applicable) and The Auth

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Damian J. Krysan,W. Scott Moye-RowleyIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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Characterizing , Pdr1, a Hyperactive Transcription Factor Involved in Azole Resistancesteps are described that lead from a characterized plasmid-borne .-GOF allele to its integration into the yeast genome in a markerless manner. Pdr1-GOF strains constructed by this approach are suitable for virulence studies in an animal host.

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