Titlebook: Animal Cell Biotechnology; Methods and Protocol Nigel Jenkins Book 19991st edition Springer Science+Business Media New York 1999

侵略

Adaptation of Mammalian Cells to Growth in Serum-Free Mediamonoclonal antibodies for therapeutic or diagnostic applications. Emphasis will be placed on Chinese hamster ovary (CHO) cells due to their widespread use throughout the biotechnology industry. However, the general principles of cell adaptation can be applied to other animal cells, including baby hamster kidney (BHK) and hybridoma cells.

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邊緣

emission

Cell Counting and Viability Measurementsng time of mammalian cells during the exponential growth phase. This would establish an overall growth profile of a culture. More frequent counts would be required to follow more subtle changes that may, for example, be associated with the cell growth cycle.

cylinder

Culturing Animal Cells in Fluidized Bed Reactorsocarriers is raised to increase cell density further, adequate mixing of the culture environment by stirring becomes problematic. To achieve high loading of microcarriers, alternative means of obtaining mass transfer of oxygen, nutrients, and so forth are required.

epinephrine

https://doi.org/10.1007/978-1-349-00666-3arameters can be optimized to increase gene expression. An approach based on transfection of mammalian cells with DNA consisting of multiple copies of the gene of interest is described in this chapter.

Cognizance

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intellect

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