Titlebook: Amino Acid Analysis; Methods and Protocol Michail A. Alterman Book 2019Latest edition Springer Science+Business Media, LLC, part of Springe

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https://doi.org/10.1007/978-3-7091-7730-3addition of isotopically labeled standard amino acids and analysis by mass spectrometry for accurate and sensitive protein quantitation. Quantitation of less than 10?fmol of protein standards with errors below 10% has been demonstrated using this method.

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https://doi.org/10.1007/978-3-7091-7730-3 be purified and their quantity and/or concentration precisely determined. In this chapter, we describe a protocol for the quantification of purified recombinant proteins. The protocol is based on a microwave-assisted acidic hydrolysis of the target protein followed by high-resolution mass spectrome