Titlebook: Agrobacterium Protocols; Volume 1 Kan Wang Book 2015Latest edition Springer Science+Business Media New York 2015 Agrobacterium-mediated tra

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Maize (, L.)ormation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an . strain carrying a standard binary vector. In addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plan

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Maize, Tropical (, L.)n staple of the diet; 95 % of the maize grown is consumed directly as human food and as an important source of income for the resource-poor rural population. The biotechnological approach to engineer biotic and abiotic traits implies the availability of an efficient plant transformation method. The

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Finger millet [, (L.) Gaertn.]we describe an optimized protocol for the .-mediated transformation of finger millet variety GPU 45. . strain LBA4404 harboring plasmid pCAMBIA1301 which contains hygromycin phosphotransferase (.) as selectable marker gene and β-glucuronidase (GUS) as reporter gene has been used. This protocol utili

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Wheat (, L.) Transformation Using Immature Embryosgrown in a well-conditioned greenhouse were pretreated with centrifuging and cocultivated with .. Transgenic wheat plants were obtained routinely from between 40 and 90 % of the immature embryos, thus infected in our tests. All regenerants were normal in morphology and fully fertile. About half of t

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