Titlebook: Affinity Chromatography; Methods and Protocol B. Vijayalakshmi Ayyar,Sushrut Arora Book 2022 Springer Science+Business Media, LLC, part of

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Affinity Chromatography978-1-0716-2176-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/978-1-0716-2176-9Fab Fragments; E; coli; protein purification; antibody; methylation

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Book 2022known pitfalls, and step-by-step, readily reproducible protocols..?Authoritative and cutting-edge,?.Affinity?Chromatography: Methods and Protocols. aims to be an invaluable resource to anyone employing affinity chromatography-based methodologies..

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The Dissolution of the Financial Statecient batch purification technique. By fusing the P-domain to a 6×His-MBP tag followed by a TEV cleavage site, we can effectively purify the P-domain in three chromatography steps (positive nickel affinity, negative nickel affinity, and negative amylose affinity).

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