Titlebook: Western Blotting for the Non-Expert; Biji T. Kurien Book 2021 Springer Nature Switzerland AG 2021 Diffusion Protein Transfer.Electrophores

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Western Blotting: How It Began,the Nobel Prize for describing the molecular structure of DNA. The genetic code had been deciphered. Nobel laureate Dr. Frederick Sanger developed a method in the early 1950s to determine the amino acid units that made up a protein structure. Now, he was set to invent an elegant method to sequence DNA in 1977.

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Sample Preparation,source for Western blotting. One can purchase purified proteins commercially, or one can purify a specific protein in the laboratory. Cells or animal tissue can serve as a crude sample source for sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Several lysis buffers are available to extract and solubilize proteins from cells or tissues.

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Things That Can Go Wrong in Western Blotting, Western blotting involves several steps beginning with making the gel. A mistake at any of these steps can affect the final result. This chapter details the problems arising during the Western blotting and subsequent immunostaining process and offers some ways to troubleshoot.

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Concentrating Proteins for Protein Blotting,on and, with appropriate methods, helps remove unwanted salts or solvents. This chapter presents several available methods to concentrate proteins in the laboratory – all described recently by Dr. Dean Goldring.

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